16S of enrichment culture: petroleum environment - SRA

SRX24462421: 16S of enrichment culture: petroleum environment
1 ILLUMINA (Illumina NovaSeq 6000) run: 75,024 spots, 33.7M bases, 11.2Mb downloads

Design: Following the centrifugation of 50 mL clay suspension at 5000g for XX minutes, Genomic DNA was extracted from the resulting pellet utilizing the Qiagen soil extraction kit (Qiagen, 47022, Valencia, CA). Subsequently, PCR amplification of hypervariable 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Submitted by: China University of Geosciences, Beijing

Study: The abiotic oxidation of petroleum hydrocarbons using reduced iron-bearing clay minerals affect the microbial degradation function.

PRJNA1108008 • SRP505874 • All experiments • All runs

show Abstracthide Abstract

Here we undertook a comprehensive study that involved both abiotic oxidation (by OH) and subsequent biological oxidation (by thermophiles in Tengchong hot spring) of petroleum hydrocarbons (contains a wide variety of hydrocarbons). These processes were facilitated by the oxidation and reduction of an iron-rich smectite, nontronite (NAu-2), respectively. Our objective was to assess the influence of altered petroleum hydrocarbons on their biodegradation mechanisms upon Fe cycling. We analyzed the Fe(II) concentration, hydrocarbons, oxygen-containing compounds, mineral transformation, bacterial community, and functional genes. These analyses were supported by wet-chemical method, mineralogy, organic geochemistry, molecular biology and metagenomics.

Sample: ArchaeaRN2-37D

SAMN41220765 • SRS21215369 • All experiments • All runs

Organism: Sphingomonadales bacterium

Library:

Name: Archaea-RN2-37D

Instrument: Illumina NovaSeq 6000

Strategy: AMPLICON

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 75,024 spots, 33.7M bases, 11.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR28905968	75,024	33.7M	11.2Mb	2024-06-08

ID:: 32778514

SRA

Sequence Read Archive

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